Effects of sustainable agronomic intensification in a forage production system of perennial grass and silage corn on nutritive value and predicted milk production of dairy cattle.

The objectives were to determine the effects of incrementally applied improved nutrient management, alternative cropping practices, and advanced production technologies in a dual forage system of perennial grass and silage corn on nutrient composition and in vitro ruminal fiber digestibility of the forages and, using these data as inputs into the Cornell Net Carbohydrate and Protein System, to predict milk production, indicators of nitrogen (N) utilization, and N excretion of dairy cattle. Farm management systems (farmlets) included a conventional system with whole manure slurry broadcast to a late maturing corn hybrid and grass harvested with 5 cuts per year (F1); improved nutrient management with a separated manure system where the sludge was applied to corn and the liquid was applied to grass (F2); improved nutrient management and alternative cropping practices with separated manure, an early maturing corn hybrid interseeded with a relay winter cover crop, and grass harvested with 3 cuts per year (F3); and improved nutrient management and alternative cropping practices combined with advanced production technologies that included irrigation and a nitrification inhibitor (F4). The field trial was a randomized complete block design over 2 yr with 4 blocks each divided into grass and corn, 4 subplots within each block for each crop, and 2 replicates within each subplot. Diets were formulation with 60% forage and 40% concentrate where the grass and corn as silage was proportional to yield for land allocations of grass and corn of 80:20, 60:40, 40:60, and 20:80. Data were analyzed using the MIXED procedure of SAS (SAS Institute Inc.). The intensified management systems (F2, F3, and F4) increased the crude protein (CP) concentration of corn with no effects on starch concentration [32.1% dry matter (DM)] compared with the conventional system (F1). Decreasing cuts of grass from 5 to 3 reduced the CP concentration in the spring harvest (15.8% vs. 12.5% DM), and increased fiber concentration and reduced digestibility in the spring, summer, and fall harvests. A common concentrate was formulated for the conventional farmlet and then combined with the forages for each farmlet within each land allocation. Forages grown under intensified management to improve N capture increased the CP concentration of the diets. However, reducing the number of cuts of grass from 5 to 3, combined with the corn and relay crop to increase yield, reduced milk production across all land allocations. To complement the nutritive value of the forages grown under each management system and land allocation, the concentrates were reformulated, which reduced dietary CP, improved the indicators of N utilization (e.g., milk urea N and milk N efficiency), reduced N excretion, and improved milk yield with no differences among the farmlets. Increasing land allocated to corn supported higher milk yield at lower dietary CP concentrations (16.5% vs. 15.4% DM) with improved milk N efficiency and lower N excretion. Intensified agronomic management increased the CP of the combined forages decreasing the need for supplemental CP in the concentrate and could reduce the importation of feed N to the farm.

Nitrogen excretion from beef cattle fed a wide range of diets compiled in an intercontinental dataset: a meta-analysis.

Manure N from cattle contributes to nitrate leaching, nitrous oxide, and ammonia emissions. Measurement of manure N outputs on commercial beef cattle operations is laborious, expensive, and impractical; therefore, models are needed to predict N excreted in urine and feces. Building robust prediction models requires extensive data from animals under different management systems worldwide. Thus, the study objectives were to 1) collate an international dataset of N excretion in feces and urine based on individual observations from beef cattle; 2) determine the suitability of key variables for predicting fecal, urinary, and total manure N excretion; and 3) develop robust and reliable N excretion prediction models based on individual observation from beef cattle consuming various diets. A meta-analysis based on individual beef data from different experiments was carried out from a raw dataset including 1,004 observations from 33 experiments collected from 5 research institutes in Europe (n = 3), North America (n = 1), and South America (n = 1). A sequential approach was taken in developing models of increasing complexity by incrementally adding significant variables that affected fecal, urinary, or total manure N excretion. Nitrogen excretion was predicted by fitting linear mixed models with experiment as a random effect. Simple models including dry matter intake (DMI) were better at predicting fecal N excretion than those using only dietary nutrient composition or body weight (BW). Simple models based on N intake performed better for urinary and total manure N excretion than those based on DMI. A model including DMI and dietary component concentrations led to the most robust prediction of fecal and urinary N excretion, generating root mean square prediction errors as a percentage of the observed mean values of 25.0% for feces and 25.6% for urine. Complex total manure N excretion models based on BW and dietary component concentrations led to the lowest prediction errors of about 14.6%. In conclusion, several models to predict N excretion already exist, but the ones developed in this study are based on individual observations encompassing larger variability than the previous developed models. In addition, models that include information on DMI or N intake are required for accurate prediction of fecal, urinary, and total manure N excretion. In the absence of intake data, equations have poor performance as compared with equations based on intake and dietary component concentrations.

Optimum roughage proportion in barley-based feedlot cattle diets: growth performance, feeding behavior, and carcass traits.

High grain diets are fed to finishing beef cattle to maximize animal performance in a cost-effective manner. However, a small amount of roughage is incorporated in finishing diets to help prevent ruminal acidosis, although few studies have examined optimum roughage inclusion level in barley-based diets. The objective of the study was to evaluate the effects of roughage proportion in barley-based finishing diets on growth performance, feeding behavior, and carcass traits of feedlot cattle. Crossbred beef steers (n = 160; mean body weight ± SD, 349.7 ± 21.4 kg) were allocated to 20 pens that were assigned randomly to four dietary treatments (five pens of eight steers per treatment). The treatment diets contained barley silage at 0%, 4%, 8%, and 12% of dietary dry matter (DM). The remainder of the diets (DM basis) consisted of 80%, 76%, 72%, and 68% barley grain, respectively, 15% corn dried distiller's grains, 5% mineral and vitamin supplement, and 32 mg monensin/kg diet DM. The diets were fed as total mixed rations for ad libitum intake (minimum of 5% refusal) once per day. Cattle were weighed on 2 consecutive days at the start and end of the experiment and on 1 d every 3 wk throughout the experiment (124 d). Two pens for each treatment group were equipped with an electronic feeding system (GrowSafe Systems Ltd., Calgary, Alberta) to monitor feed intake and feeding behavior of individual cattle. The data for dry matter intake (DMI), average daily gain (ADG), gain:feed (G:F) ratio, and carcass traits were analyzed as a completely randomized design with fixed effect of barley silage proportion and pen replicate as experimental unit. Feeding behavior data were analyzed similarly, but with animal as experimental unit. Averaged over the study, DMI increased linearly (11.1, 11.3, 11.7, 11.8 kg/d; P = 0.001) as barley silage proportion increased from 0%, 4%, 8%, and 12% of DM, but ADG was not affected (carcass-adjusted,1.90, 1.85, 1.87, 1.89 kg/d; P ≥ 0.30). Consequently, G:F ratio decreased linearly (carcass-adjusted, 168.9, 163.8, 158.5, 160.6 g/kg DMI; P = 0.023). When averaged over the study, proportion of barley silage in the diet had no linear or quadratic effects (P > 0.10) on meal frequency, duration of meals, intermeal duration, or meal size, but eating rate decreased linearly with increasing silage proportion (P = 0.008). There was no diet effect on liver abscesses (P ≥ 0.92), and effects on carcass characteristics were minor or nonexistent. We conclude that increasing the proportion of barley silage in a feedlot finishing diet at the expense of barley grain to minimize the incidence of ruminal acidosis may decrease feed conversion efficiency.

Optimum roughage proportion in barley-based feedlot cattle diets: total tract nutrient digestibility, rumination, ruminal acidosis, short-chain fatty absorption, and gastrointestinal tract barrier function.

Cattle need physically effective fiber to promote rumination and maintain rumen health, but economics favor the use of low-roughage feedlot diets. The study investigated the optimum barley silage proportion in barley-based finishing diets. Apparent total-tract digestibility (4-d total fecal collection), chewing behavior (6-d video recording), ruminal pH (6-d indwelling pH recording), and fermentation (1 day, sampling 0, 3, 6, 12, and 18 h postfeeding), short-chain fatty acid (SCFA) absorption (washed reticulo-rumen technique), gastrointestinal tract barrier function (marker infusion), and blood variables (catheters) were measured. Eight ruminally fistulated crossbred beef heifers (653 ± 44.2 kg; mean starting body weight [BW] ± SD) were used in a replicated 4 × 4 Latin square design with 28-d periods. Dietary treatments were 0%, 4%, 8%, and 12% of dietary dry matter (DM) as barley silage, with diets containing 80%, 76%, 72%, and 68% barley grain, respectively. Increasing silage proportion decreased dietary starch content from 49.0% to 43.1% DM, while neutral detergent content increased from 22.7% to 25.1% DM. Silage proportion had no effect on DM intake, but apparent DM digestibility decreased quadratically (86.0%, 82.1%, 81.1%, 79.5% for the four diets, respectively; P < 0.001). Although, silage proportion had no effect on eating activity, rumination time increased quadratically (246, 289, 302, 316 min/d; P = 0.04). Increased silage proportion increased minimum (5.07, 5.27, 5.29, 5.41; quadratic, P = 0.011) and mean (5.61, 5.87, 5.93, 5.95; quadratic, P = 0.007) ruminal pH, and there was a quadratic (P ≤ 0.047) decrease in duration and area under the pH acidosis threshold curves of 5.8, 5.5, and 5.2. Although increasing silage proportion decreased ruminal acidosis, it was not completely eliminated even with a diet containing 12% silage DM. SCFA concentration in ruminal fluid was not affected by diet, but silage proportion quadratically (P ≤ 0.088) increased ruminal acetate:propionate. There was no effect of diet on absolute or fractional rates of absorption of acetate, propionate, butyrate or total SCFA, and no effect on gastrointestinal barrier function or blood measurements. In conclusion, responses to roughage level were mostly quadratic with greatest improvements in acidosis variables between 0% and 4% barley silage, with incremental improvements with further increases in silage levels. The study showed a trade-off between maximizing digestibility and energy intake to promote animal performance and minimizing the risk of acidosis.

Use of gallic acid and hydrolyzable tannins to reduce methane emission and nitrogen excretion in beef cattle fed a diet containing alfalfa silage1,2.

The objective of this study was to determine the effects of different forms of hydrolyzable tannin [HT; source (chestnut, CN; tannic acid, TA); subunit (gallic acid, GA)] on apparent total-tract digestibility, methane (CH4) production, and nitrogen (N) utilization in beef cattle fed an alfalfa silage-based diet. Eight ruminally cannulated heifers with an initial BW of 480 ± 29.2 kg (mean ± SD) were used in a double 4 × 4 Latin square experiment. The experiment consisted of four 28-d periods (14-d adaptation, 14-d measurements) and a 7-d washout between periods. The animals received a basal diet with 19.8% CP (DM basis) content containing 75% alfalfa silage, 20% barley silage, and 5% supplement (DM basis) with or without different forms of HT. The dietary treatments were as follows: control (no HT), GA (1.5% of diet DM), TA (1.5% of diet DM), and CN (2% of diet DM). Animals were fed 95% of their ad libitum intake during the measurement phase. Total fecal excretion was collected for 4 d, CH4 was measured for 72 h using respiration chambers, and ruminal fermentation variables and plasma urea N (PUN) concentration were measured on 2 nonconsecutive days before and after feeding. The restricted DM (DMI; 10.79 ± 1.076 kg/d) and nutrient intakes did not differ (P ≥ 0.22) among treatments. Furthermore, apparent DM digestibility (60.3 ± 0.86%) was not affected (P = 0.20) by treatment, but CP digestibility decreased for TA and CN compared with control and GA treatments (63.1 vs. 69.0%; P < 0.001). Total VFA concentration tended (P = 0.089) to increase for GA compared with control and TA (134 vs. 125 and 126 mM) and intermediate for CN (129 mM). The PUN concentration was lower for all HT treatments compared with control (196 vs. 213 mg/L; P = 0.02). Both TA and CN increased the proportion of N excreted in feces and decreased the proportion in urine compared with control and GA (43.9% vs. 37.8% and 56.1% vs. 62.2%; respectively; P < 0.001). However, the proportion of urea N in urinary N decreased for all HT treatments compared with control (47.2% vs. 51.2%; P = 0.02). Also, GA tended to decrease CH4/DMI (20.4 vs. 22.3 g/kg DMI; P = 0.07) and decreased the proportion of GE intake emitted as CH4 (5.16 vs. 5.71%; P = 0.04) compared with control. Thus, among the different forms of HT applied to a high-protein alfalfa silage-based diet, both TA and CN had no effect on CH4 production, but decreased CP digestibility and shifted N excretion from urine to feces, whereas GA (i.e., HT subunit) decreased CH4 production and decreased the proportion of urea N in urinary N in beef cattle without affecting CP digestibility. Thus, feeding the HT subunit, GA, has the potential to decrease environment impact of ruminants (lower CH4 and ammonia emissions), without decreasing animal performance.

Effects of hydrolyzable tannin with or without condensed tannin on methane emissions, nitrogen use, and performance of beef cattle fed a high-forage diet.

Sustainability of animal agriculture requires efficient use of energy and nitrogen (N) by ruminants fed high-forage diets. Thus, there is a need to decrease methane (CH4) emissions and prevent excessive N release into the environment. Therefore, this experiment examined the long-term effects of feeding hydrolyzable tannin (HT) with or without condensed tannin (CT) on animal performance, rumen fermentation, N use, and CH4 production in beef cattle fed a high-forage diet. A total of 75 weaned crossbred steers (292 ± 4.1 kg) were grouped by body weight (BW), housed in individual pens, and randomly assigned to 1 of 5 dietary treatments (15 animals/treatment) in a completely random design. The animals were fed a basal diet of alfalfa:barley silages (50:50; dry matter [DM] basis) with a crude protein content of 17.1% and supplemented with HT extract (chestnut, CN) or a combination (50:50) of HT and CT extracts (quebracho, Q) in a powdered form at different levels of dietary DM. The treatments for determining animal performance and N use were control (no tannin), 0.25% CN, 1.5% CN, combination of CN and Q at 0.125% each (0.25% CNQ), and CN and Q at 0.75% each (1.5% CNQ) of dietary DM. The treatments for the CH4 measurement were control, 1.5% CN, and 1.5% CNQ of dietary DM. The first 84 d of the study were used to measure animal performance, rumen fermentation, and N use, and the next 30 d were used to measure CH4 emissions with the tracer gas technique. There were no effects of treatment on DM intake (DMI), BW, average daily gain, and gain: feed (P ≥ 0.10). The plasma urea N concentration was greater (P < 0.05) for 1.5% CN and 1.5% CNQ than those fed 0.25% CNQ (120.9 and 120.4 vs. 111.7 mg/L, respectively), but not different (P > 0.05) from animals fed control or 0.25% CN (117.2 and 117.5 mg/L, respectively). Tannin inclusion did not affect rumen pH, total volatile fatty acid concentration, proportions of acetate and propionate, and total protozoa populations (P ≥ 0.16). Tannin, irrespective of type or dose, decreased (P < 0.01) ruminal ammonia concentration. Tannin type and dose did not affect (P = 0.54) daily CH4 production (154 ± 5.9 g/d) but 1.5% CNQ tended to decrease CH4 yield compared with control (20.6 vs. 22.0 g/kg DMI; P = 0.094). HT from CN alone or in combination with CT from Q can be added at a low (0.25% DM) or high (1.5% DM) level to a forage-based diet to decrease ruminal ammonia concentration in growing beef cattle fed a high-protein diet without adverse effects on animal performance. A combination of HT and CT at a concentration of 1.5% dietary DM also tended to decrease CH4 emissions without negatively affecting performance.

Feeding condensed tannins to mitigate ammonia emissions from beef feedlot cattle fed high-protein finishing diets containing distillers grains.

Two experiments were conducted to determine the effects of feeding a condensed tannin extract (CT) on dry matter intake (DMI), growth performance, carcass traits, and NH3-N emissions of beef feedlot cattle fed high-protein barley-based finishing diets. In Exp. 1, 36 crossbred steers (346 ± 4.2 kg) were individually fed 4 diets with 20% corn dried distillers grains (DG) and increasing concentrations of a CT extract from Acacia mearnsii (black wattle) at 0%, 1.2%, 2.4%, and 3.5% of DM (9 steers per diet) for 52 d. The DMI was not affected at 1.2% and 2.4% but tended (P = 0.08, quadratic effect) to decrease at 3.5% CT extract. There was no effect (P ≥ 0.12) of increasing CT extract on ADG, but G:F tended (P = 0.09) to decrease linearly. In Exp. 2, 148 crossbred steers (457 ± 3.8 kg) were allocated to 16 pens with 4 pens per treatment in a completely randomized design and fed for 83 d. The 4 dietary treatments included 0% corn DG (0DG), 20% DG (20DG), 40% DG (40DG), and 40% DG with 2.5% CT extract (40DGCT) and contained 13.3, 15.9, 20.4, and 19.4% CP, respectively. All cattle were weighed, and blood was collected from 5 steers per pen every 3 wk. Ammonia emissions were measured in four 3-wk periods using the integrated horizontal flux technique with passive NH3 samplers from 2 pens of cattle fed 0DG and 20DG (Period 1), 40DG and 40DGCT (Period 2), 0DG and 40DG (Period 3), and 0DG and 40DGCT (Period 4). There was no effect (P ≥ 0.15) of diet on final body weight (621 ± 7.1 kg), DMI (11.9 ± 0.25 kg/d), ADG (1.98 ± 0.07 kg/d), G:F (166 ± 5.4 g/kg), and carcass traits. Plasma urea N (PUN) increased (P < 0.001) from 0DG to 40DG (113 to 170 ± 6.0 mg N/L) and was reduced (P < 0.001) by 40DGCT (146 mg N/L) compared with 40DG and tended (P = 0.09) to be reduced compared with steers fed 20DG (153 mg N/L). Ammonia-N emissions were greater from cattle fed 40DG [113.7 vs. 70.8 ± 4.57 g N/(steer·d), P = 0.003] and tended to be greater from cattle fed 20DG [51.3 vs. 26.3 ± 11.2 g N/(steer·d), P = 0.11] compared with 0DG. Cattle fed 40DGCT tended to have lower NH3-N emissions compared with cattle fed 40DG [72.7 vs. 95.1 ± 9.3 g N/(steer·d), P = 0.09 and 20.5 vs. 26.5 ± 2.64% N intake, P = 0.11]. Feeding 2.5% CT to beef feedlot cattle fed a high-protein diet had no detrimental effect on performance, reduced PUN indicating lower urinary urea N excretion, and lowered NH3-N emissions by 23%.

Effect of feeding condensed tannins in high protein finishing diets containing corn distillers grains on ruminal fermentation, nutrient digestibility, and route of nitrogen excretion in beef cattle.

Eight ruminally cannulated crossbred beef heifers (427 ± 41.2 kg, body weight) were used in a replicated 4 × 4 Latin square to determine the effects of feeding a condensed tannin (CT) extract with high protein diets containing corn dried distillers grains and solubles (DG) on ruminal fermentation, nutrient digestibility, and route of nitrogen (N) excretion. Dietary treatments included [dry matter (DM) basis]: 0 (0DG), 20 (20DG), and 40% DG (40DG), and 40% DG with 2.5% CT extract (1.33% CT) from Acacia mearnsii (40DGCT). The DG and CT extract were substituted for grain in a barley-based diet that contained 91% concentrate and 9% silage (DM basis) and was fed as a total mixed ration once daily. The crude protein concentrations of the diets were 12.9, 16.8, 20.4, and 20.5% for 0DG, 20DG, 40DG, and 40DGCT, respectively. Periods were 5 wk with 2 wk for transition to the DG level of the diets, 1 wk for adaptation to CT, and 2 wk for measurements. Feed offered and refused were measured daily. Total urine and fecal output were collected daily for 4 d consecutively. Data were analyzed using a mixed linear model with diet and period as fixed effects and square and animal within square as random effects. There was no effect (P ≥ 0.22) of CT on DM intake, but 40% DG in the diet (40DG and 40DGCT) decreased (P ≤ 0.015) DM intake compared with 20DG. As a result, nitrogen (N) intake was not different (P > 0.15) among heifers fed 20DG, 40DG, and 40DGCT (313 g N/d) and was less (P ≤ 0.001) for heifers fed 0DG (220 ± 18 g N/d). Apparent total tract N digestibility was less (P ≤ 0.001) in heifers fed 40DGCT (70.6 ± 1.07%) compared with to 0DG, 20DG, and 40DG (78.4%). There was no effect (P = 0.84) of CT (40DGCT vs. 40DG) on the total N output, however, feeding 40DGCT decreased (P ≤ 0.001) the excretion of total urinary N and urea N in urine by 17 and 21%, respectively, compared with heifers fed 40DG and was equivalent (P ≥ 0.12) to the amount excreted by heifers fed 20DG. The reduction of N digestibility reflected the protein binding effects of CT within the gastrointestinal tract and the shift in excess N excretion from labile urea N in urine to bound NDIN and ADIN in feces (P ≤ 0.001) in heifers fed 40DGCT compared with 40DG. Supplementation of CT in high protein diets fed to feedlot cattle reduced urinary N and increased the capture of N in manure to potentially lesson the loss of N as ammonia and provide opportunities for improved nutrient management of beef production.

Effects of Feeding Encapsulated Nitrate to Beef Cattle on Ammonia and Greenhouse Gas Emissions from Their Manure in a Short-Term Manure Storage System.

A study was conducted to investigate effects of feeding encapsulated nitrate (EN) to beef cattle on ammonia (NH) and greenhouse gas emissions from their manure. Eight beef heifers were randomly assigned to diets containing 0 (control), 1, 2, or 3% EN (55% forage dry matter; EN replaced encapsulated urea in the control diet and therefore all diets were iso-nitrogenous) in a replicated 4 × 4 Latin square design. Urine and feces collected from individual animals were reconstituted into manure and incubated over 156 h using a steady-state flux chamber system to monitor NH, methane (CH), carbon dioxide (CO), and nitrous oxide (NO) emissions. Urinary, fecal, and manure nitrate (NO)-N concentration linearly increased ( < 0.001) with feeding EN, and urinary urea concentration tended to be lower ( = 0.078) for EN versus Control. The hourly emissions of NH, CO, and NO (mg head h) were not affected, although NH emission rates tended to be lower ( = 0.070) for EN compared with Control at 0 to 12 h. Cumulative NH, CO, and NO emissions over 156 h were not affected, but CH emissions were less (4.5 vs. 7.4 g head; = 0.027) for EN compared with Control. In conclusion, although NH emissions were initially lower for EN manures, total NH emitted over 156 h was not affected. Dietary EN lowered CH emissions from manure, and, despite greater NO concentrations in EN manure, NO emissions were not affected in this short-term incubation.